List of Abstracts

Domain communications within the global bacterial Rcs signaling network
Poster plus application for short presentation
Signaling networks, Cell Communication and Signaling , The atomic view of signaling
Sialic acid (biosynthesis) is involved in gene expression, proliferation and cell differentiation
Invited Speaker
Cause and consequence of altered mitochondrial dynamics in mitochondrial complex I deficiency
Poster plus application for short presentation
Mitochondria are critically involved in cell cycle regulation, apoptosis, calcium signaling, organismal development, immune responses and dynamic modulation of respiratory capacity. In this way, the relevance of mitochondria for proper cell function by far exceeds their role as ATP generators. This places mitochondrial (dys)function not only at the heart of normal cell function but also implicates this organelle in the etiology of human disorders including diabetes, genetic oxidative phosphorylation defects, and neurodegenerative disorders such as certain forms of Parkinson and Alzheimer’s disease. Our research aims to understand the interconnection between mitochondrial dynamics and function at the molecular and life cell level. As a model system we use dermal fibroblasts and cancer cell lines in which a deficiency in mitochondrial complex I (CI) is chemically induced as well as cells of children with inherited mitochondrial disorders.
Pasteurella multocida toxin activates heterotrimeric G proteins by deamidation
Poster plus application for short presentation
A large scale Split-Ubiquitin screen to monitor protein-protein interactions involved in cytokinesis
Poster presentation
other free topics
MODULATION OF DENDRITIC CELL FUNCTION BY SCHISTOSOME GLYCOCONJUGATES
Invited Speaker
c-Cbl-dependent FLIPS degradation pathway and its role in Mycobacterium tuberculosis-induced macrophage apoptosis
Poster plus application for short presentation
Signaling in Disease
cAMP-induced phosphorylation and 14-3-3-binding controls subcellular localization of SIK2 in adipocytes
Poster plus application for short presentation
Signaling networks
We investigated the regulation of the AMPK-related kinase SIK2 in adipocytes. We believe that regulation of SIK2 by cAMP may be important for the effects of this second messenger on lipid metabolism.
Endosomal adaptor proteins APPL1 and APPL2 are novel activators of β-catenin/Tcf-mediated transcription
Poster plus application for short presentation
Signaling networks
Redox-sensitive cysteines bridge p300/CBP-mediated acetylation and FoxO4 activity
Poster plus application for short presentation
Signaling networks
Toll-like receptor signal transduction in immunity and inflammation
Invited Speaker
Signaling in Disease
Toll-like receptors
TGF-beta receptor signaling in angiogenesis
Invited Speaker
Signaling in Disease
Genetic studies in mice and humans have revealed an important role for TGF-beta in vascular development and maintenance. We have investigated the molcular mechanisms in which misregulation of TGF-beta signaling may contribute to vascular disorders and tumor angiogenesis.
Lgr5 Intestinal Stem Cells in self-renewal and cancer
Invited Speaker
other free topics
Hans Clevers Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences & University Medical Centre Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands
Activation of Protein Kinases by the Hsp90 Molecular Chaperone System
Invited Speaker
Signaling networks
SELECTIVE AUTOPHAGY - CLEARANCE OF AGGREGATED PROTEINS AND DYSFUNCTIONAL ORGANELLES
Invited Speaker
other free topics
An expression module of WIPF1-coexpressed genes identifies patients with favorable prognosis in three tumor types
Poster plus application for short presentation
other free topics
I would like to present our recent work on the identification of a prognostic microarray-based gene expression signature for colorectal, brain & breast cancers that is based on genes that are co-expressed with the WIPF1 gene (published in June in JMM; see below). My hope is that a possible presentation will lead to contacts with groups working on cytoskeleton regulation that eventually will become interested in the bridge to cancer development and want to explore the functional aspects of this new diagnostic gene set. I note that this is precompetive research and none of the employers of the authors has indicated financial interests because none of them is developing commercial diagnostics. The work will also be presented at the Biotechnika / Bioinformatics track in October 2009.
Signaling via Anaplastic Lymphoma Kinase in Drosophila
Invited Speaker
Signaling networks
Constitutive Activity of GPCRs : role in physiology, evolution and diseases
Invited Speaker
Signaling in Disease
Distinct negative feedback loops regulate WCC-dependent transcription in Neurospora crassa
Poster plus application for short presentation
Signaling networks, Biophysical aspects of signal transduction
We show how attenuation of light dependent gene expression in Neurospora (photoadaptation) is mediated by the blue-light receptor VIVID.
Stimulus context erases pain sensitization
Invited Speaker
Signaling in Disease, Signaling networks
The outcome of a stimulus is dependent on the signalling history of the cell. We observed that the context can even invert the expected outcome.
Distinct regulation of the reduced folate carrier transport activity by xenobiotics via nuclear receptor signalling pathways
Poster plus application for short presentation
Xenobiotics and Signalling
Data on the molecular mechanisms how xenobiotics influence the carrier associated transport of endogenous substances and drugs resulting in drug-drug-interactions and the alteration of the physiological homoeostasis.
Structural analysis of multi-protein signalling complexes - the divide and conquer approach
Invited Speaker
Cell Communication and Signaling
Gab proteins are essential assembly platforms for complex molecular machines which integrate and distribute important physiological signals, but can also support the development of several cancer types. Recent biophysical data and multi-genome comparisons allow new insight into the building principles and actions of these molecular computing units and point to new strategies for future cancer therapies.
A New Strategy against RGD-related Diseases
Poster presentation
Signal Transduction and Drug Discovery
Astrocyte dysfunctions in Parkinson`s disease
Poster plus application for short presentation
Proteomics, Signalling and Disease
Nitric Oxide enhances plasma membrane fluidity: a novel mechanism for morphological differentiation?
Poster presentation
Cell Communication and Signaling , Biophysical aspects of signal transduction
Zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
Poster plus application for short presentation
Stem Cells and Growth Control , Cell Communication and Signaling
Characterisation of novel glycan-binding receptors and their interactions with intracellular signalling pathways
Invited Speaker
Glycobiology and Cell Signalling
This is an invited lecture that will attempt to link carbohydrate recognition processes at the cell surface with intracellular signalling.
Beyond the role in endocytosis: a novel function of clathrin in NF-kappaB pathway discovered from an RNAi screen
Poster presentation
Signaling in Disease
MUSCARINIC AGONIST, AND ANTAGONIST AND INHIBITORS TREATMENT CHANGES IN c-FOS AND CYCLIN D1 TRANSCRIPTS
Poster presentation
Cell Communication and Signaling
Muscarinic receptor mediated cell signalling in K562 erythroleukemia cell line
Cooperation of NF-kB and CREB to induce synergistic IL-6 transcription upon combined triggering of β2-adrenergic and TNF-receptors in astrocytes.
Poster plus application for short presentation
Signaling networks
Specific induction of migration and invasion of pancreatic carcinoma cells by RhoC which differs from RhoA in its localisation and activity
Poster plus application for short presentation
Xenobiotics and Signalling
Nrf2 induces IL-6 expression via an antioxidant response element within the IL-6 promoter
Poster plus application for short presentation
Signaling in Disease, Signaling networks
Monocyte activation by Toll-like receptor 4 depends on zinc signals
Poster plus application for short presentation
other free topics
Zinc ions are released intracellularly after TLR4-stimulation (independent from MyD88 or TRIF)and inhibit phosphatases. This is a physiological signal involved in activation of signaling pathways and cytokine production
Targeting JAK/STAT signaling pathways and polo-like-kinases by small-molecule inhibitors of protein-protein interactions
Invited Speaker
Signal Transduction and Drug Discovery
Identification of putative miR-21 targets in LNCaP prostate cancer cells
Poster presentation
Proteomics, Signalling and Disease
The role of c-Abl in Helicobacter pylori-infected epithelial cells
Poster presentation
Signaling in Disease
Exploring TNF-α and cAMP–induced signaling pathways regulating IL-6 expression in skeletal muscle cells
Poster presentation
Signaling networks
The IL-15/IL-15R signaling pathway
Invited Speaker
Signaling networks
Lef-1 splice variants induce invasion or proliferation of pancreatic cancer cells
Poster plus application for short presentation
Signaling in Disease
We have characterised a second Lef-1 isoform which lacks exon VI. We show for the first time that the two Lef-1 isoforms modulate the expression of different genes leading to cell proliferation in the case of full length Lef-1 and to cell migration in case of the new Lef 1 isoform which lacks exon VI. Moreover, we correlate the increased expression of the isoforms of the transcription factor Lef-1 in pancreatic carcinoma with increased tumour progression in vivo and increased cell migration in vitro. The discovered relation between Lef-1 isoforms and the control of varying sets of target genes will help to understand the diverse effects of Lef-1 in carcinogenesis.
O-Mannosylation: Cis regulation of a highly protein-specific glycosylation
Poster presentation
Glycobiology and Cell Signalling
THE HUMAN PrRP RECEPTOR: STRUCTURE-ACTIVITY RELATIONSHIPS
Poster presentation
Signal Transduction and Drug Discovery
Jaks, STATs, and their target genes in health and disease
Invited Speaker
Cell Communication and Signaling
Functional and proteomic characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase
Invited Speaker
Proteomics, Signalling and Disease
Qualitative and quantitative analysis of mitochondrial population in 3T3-L1 adipocytes responding to a mild mitochondrial uncoupling: a mitoproteomic approach
Invited Speaker
Proteomics, Signalling and Disease
Smif (Smad4 interacting factor) is a negative regulator of T-cell activation and autoimmunity in vivo
Poster plus application for short presentation
Signaling in Disease
Insights into the delta-opioid receptor signaling network
Poster presentation
Signaling networks
Structural basis for the membrane-association of the redox-sensitive TOR FATC domain
Poster plus application for short presentation
Biophysical aspects of signal transduction
THOC5/FMIP, an mRNA TREX export complex protein and a substrate for leukemic tyrosine kinases, is essential for hematopoietic stem cell survival in vivo
Poster plus application for short presentation
Stem Cells and Growth Control
We have identified THOC5/ Fms interacting protein (FMIP/THOC5), a member of mRNA export complex TREX as a substrate for leukemic tyrosine kinases. To study the function of FMIP/THOC5 in vivo, we generated for the first time the interferon inducible knockout mouse and demonstrated that this gene is essential for hematopoietic stem cell survival.
Cellular migration and invasion – Signal transduction pathways induced by Helicobacter pylori
Invited Speaker
Cell Communication and Signaling
Syndecan-1, the primary hepatic proteoglycan receptor for triglyceride-rich lipoprotein clearance
Invited Speaker
Glycobiology and Cell Signalling
Protein Dysglycosylation as a pathomechanism of chronic cell damage in classical galactosemia
Poster plus application for short presentation
Glycobiology and Cell Signalling
Galactosemia a lethal disease, caused by the enzyme galactose-1-phosphate uridyltransferase and disglycosylation dependency
Alpha-Skeletal Muscle Actin Nemaline Myopathy Mutants cause cell death in Cultured Muscle Cells
Poster presentation
other free topics
Metabotropic Glutamate Receptors: complex allosteric machines for new drugs
Invited Speaker
Cell Communication and Signaling
A highly potent transcriptional regulator is out-of-frame translated from the metallothionein 2A messenger RNA
Poster plus application for short presentation
other free topics
Protein-Protein interaction.
Hsps 70 and 105 associate with a group of hsp90 client proteins that are selectively found in human breast cancer
Poster plus application for short presentation
Signaling in Disease
Qualitative and quantitative analysis of mitochondrial population in 3T3-L1 adipocytes responding to a mild mitochondrial uncoupling: a mitoproteomic approach
Poster presentation
Proteomics, Signalling and Disease
Tumor suppressor genes required for chromosomal stability
Poster plus application for short presentation
Signaling in Disease
We identified tumor suppressors required for chromosomal stability. Loss of these genes in human cancer cells causes mitotic defects and aneuploidy that might directly contribute to tumorigenesis.
Effect of intermittent hypoxia on Nrf2 and NF-κB activity in endothelial cells
Poster presentation
other free topics
Hereditary retinal cone dystrophy linked with mutations in a Ca2+-sensor
Poster presentation
Signaling in Disease
The role of PrPC and its pathological isoform PrPSc in cellular signalling pathways
Poster presentation
Proteomics, Signalling and Disease
Peroxynitrite activates the transcription factor Nrf2 in human endothelial cells.
Poster presentation
Cell Communication and Signaling
The Anterograde Transport of the Adiponectin Receptor 1 to the Plasma Membrane is Regulated by Specific Sequence Motifs in the Receptor N-Terminus
Poster presentation
Cell Communication and Signaling
Functional analysis of the Nod1 and Nod2 NACHT domain reveals differences in their ability to activate NF-kB
Poster plus application for short presentation
Signaling networks
Identification of chemical inhibitors of Nod2 signalling
Poster presentation
Signal Transduction and Drug Discovery
Sulforaphane and Selenium in Inflammation and Phase II Enzyme Expression
Poster plus application for short presentation
Signaling in Disease
Investigations on the molecular mechanism of a muscular dystrophy (FSHD) by proteomic and metabonomic analyses of primary myoblast cultures
Invited Speaker
Proteomics, Signalling and Disease
In order to better understand the molecular mechanism of a muscular dystrophy, we have studied primary muscle cells derived from biopsies of patients and non affected individuals by proteomics and metabonomics approaches.
STAT3 is a determinant of malignancy in solid cancers
Invited Speaker
Cell Communication and Signaling
Role of Wnt/β-catenin signaling for expression and inducibility of drug-metabolizing enzymes in liver
Invited Speaker
Xenobiotics and Signalling
The Role of Extra- and Intracellular Domains for Y Receptor Internalisation
Poster presentation
Signaling networks
The interplay between phosphorylation and ubiquitination in regulating the innate immune system
Invited Speaker
Signal Transduction and Drug Discovery
Approaches to the identification of regulatory determinants in glycoproteins controlling the LacdiNAc-modification of O-linked glycans
Poster presentation
Glycobiology and Cell Signalling
Cis-Control of Terminao O-Glycan Modification by Peptidic Elements
Quantitative dynamic proteome biology of anterior mice hippocampus
Poster plus application for short presentation
Proteomics, Signalling and Disease
Using a mouse model of induced hippocampal cell loss, dynamic protein expression analyses were conducted at different time-points.
Differential effects of multiplicity of infection on Helicobacter pylori induced signaling pathways and interleukin-8 gene transcription
Poster plus application for short presentation
Signaling in Disease
Insulin resistance of keratinocytes contributes to the manifestation of the psoriatic phenotype
Poster presentation
Signaling in Disease
TLR4-mediated IL-6 gene expression in macrophages: who does the job?
Poster plus application for short presentation
Signaling networks
IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR
Poster plus application for short presentation
Proteomics, Signalling and Disease
Employing RNA affinity chromatography IGF2BP1 (ZBP1)has been identified as an HCV mRNA 5'UTR (HCV IRES) and -3'UTR interacting protein, which enhances HCV IRES-mediated translation initiation in an 3'UTR dependent manner.
MEMBRANE RECYCLING AND EXOSOMAL EXPORT OF MUC1 CORRELATE WITH DIFFERENTIAL O-LINKED OR N-GLYCOSYLATION
Poster presentation
Glycobiology and Cell Signalling
Recycling of membrane protein (MUC-1), O- and N-glycosylation, exosomal targeting
DDX6 is a novel regulator of reticulocyte 15-lipoxgenase mRNA translation
Poster plus application for short presentation
Proteomics, Signalling and Disease
Modulation of FoxO signaling by polycyclic aromatic hydrocarbons
Poster presentation
Xenobiotics and Signalling
SH2 domains, tyrosine kinases and signaling networks
Invited Speaker
Proteomics, Signalling and Disease
The mechanisms by which complexity is acquired at the protein and cellular level will be discussed.
Identification of Novel Binding Partners of TrkA
Poster plus application for short presentation
Proteomics, Signalling and Disease
Isolotion of novel TrkA substrates using a constitutively active fusion protein of LexA and intracellular domain of TrkA and identification by mass spectrometry
Zinc signals in Interleukin-2-receptor signaling
Poster presentation
other free topics
Selective requirement of p65 phosphorylation in the regulation of specific NF-kB-dependent gene expression
Poster presentation
Signaling in Disease, Signaling networks, Signal Transduction and Drug Discovery
Characterization of harmine as a potent and specific inhibitor of the protein kinase DYRK1A
Poster presentation
Signal Transduction and Drug Discovery
Signaling via receptor for PDGF and TGF-beta - possible targets in tumor therapy
Invited Speaker
Signaling in Disease
KOX 15 controls proliferation of medulloblastoma cells and predicts patients’ survival
Poster presentation
Stem Cells and Growth Control
Zinc finger transcription factor KOX15 (ZNF22) plays important role in growth of medulloblastoma and might be used as a new prognostic marker for medulloblastoma patients
Far-field fluorescence STED microscopy reveals details of nanoscale membrane dynamics
Invited Speaker
Biophysical aspects of signal transduction
Adipokine Expression to Investigate Structure and Function of Vaspin and Adiponectin
Poster presentation
Cell Communication and Signaling
Copper- and myeloperoxidase-modified low-density lipoproteins activate the transcription factor Nrf2 through different pathways of ROS production in macrophages
Poster plus application for short presentation
Signaling in Disease
Biochemical Characterization of DYRK4
Poster presentation
other free topics
PA28g–dependent anti-apoptotic Signalling in UV-treated Fibroblasts
other free topics, Signaling in Disease, Signaling networks, Signal Transduction and Drug Discovery
ATP-dependent stabilization of fibroblast growth factor 2
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Site-directed mutagenesis within the heparin binding domain abolishes ATP-binding and neuroprotective effect of nerve growth factor
Poster plus application for short presentation
other free topics
Cardiac hypertrophy induced by IL-6-type cytokines – a fine line between protection and injury
Poster presentation
Signaling in Disease
Aggravation by prostaglandin E2 (PGE2) of interleukin-6 (IL-6)-dependent insulin resistance in hepatocytes
Poster plus application for short presentation
Signaling in Disease
Cytokines and prostanoides modulate insulin signaling in hepatocytes and might contribute to hepatic insulin resistance.
Substrate specificity of protein histidine phosphatase
Poster plus application for short presentation
other free topics
Attenuation of Hepatic Expression and Secretion of Selenoprotein P by Metformin
Poster presentation
Xenobiotics and Signalling
A positive correlation of high serum selenium levels with the prevalence of type 2 diabetes has recently been found. Selenoprotein P (SeP), which is secreted by hepatocytes, is the major selenoprotein in plasma. The aim of this study was to investigate whether the antidiabetic drug metformin affects hepatic expression and secretion of selenoprotein P.
The transmembrane domain of PDGFRβ is crucial for the activation of the TEL-PDGFRβ hybrid cytosolic oncoprotein
Poster plus application for short presentation
Signaling in Disease
Towards a Systems Biology Analysis of Adaptor protein-regulated signaling in Bcr/Abl+ Leukemia.
Poster presentation
Signaling networks
Inhibition of FOXO gene expression by growth factors
Poster plus application for short presentation
Signaling networks
A new mechanism of FOXO regulation
RAC1 SIGNALING MODULATES BCL-6-MEDIATED REPRESSION OF GENE TRANSCRIPTION
Poster presentation
Signaling networks
Altered ß-adrenergic answer due to a cardiomyopathy inducing mutation in the cardiac troponin I gene
Poster presentation
other free topics, Signaling in Disease
NNegative regulation of Raf kinase signaling by PDZ proteins
Poster plus application for short presentation
Signaling networks
Kinases as Raf, Src, Bcr are involved in growth, we analyzed antiproliferative effects by tumor suppressor PDZ-domain proteins.
Axonal mRNAs: Characterisation and role in the growth and regeneration of dorsal root ganglion axons and growth cones
Poster presentation
other free topics
Neurons transport mRNAs along their axons. Here we characterised the axon species present in peripheral axons and we determined a role for local translation in growth cone regeneration
Is voltage-dependent anion channel 1 (VDAC-1) involved in Ha-Ras-mediated neuronal protection?
Poster plus application for short presentation
Signaling in Disease
Intracellular retention of FLT3 and signaling quality
Poster plus application for short presentation
Signaling in Disease
Ras homologue enriched in brain (Rheb) enhances apoptosis
Poster presentation
Signaling in Disease
A kinetic approach identifies target sites and synergistic effects of drugs – potentiated inhibition of glycine receptor channels by flavonoids and strychnine
Poster plus application for short presentation
Signal Transduction and Drug Discovery
A mechanismßbased approach is presented that alows the delineation of inhibition mechanisms, and whether two drugs bind to th same, or different sites on their target protein. The general mechanism is applied to inhibition of the glycine receptor, a ligand-gated ion channel, bz flavonoidas and strychnine. Indeed, strychnine toxicity is increased in votro and in vivo in the presence of flavonoids. Thus, a method to assess the combined effedts of multiple drugs has been established
AEZS-126, a new orally bioavailable PI3K inhibitor with antitumor effects
Poster presentation
Signaling in Disease, Signal Transduction and Drug Discovery
A novo specific 5-HT2B-receptorantagonist for the prophylactic treatment of migraine
Poster plus application for short presentation
Proteomics, Signalling and Disease
Characterisation of Chemerin and its Receptors with Respect to Obesity
Poster presentation
Cell Communication and Signaling
Lentiviral infection and differentiation of embryonic stem cells: A cell culture model for studying the influence of HRP-3 on early neural development
Poster presentation
Stem Cells and Growth Control
Relevance of the adaptor protein Shc in Oncostatin M signal transduction
Poster plus application for short presentation
Signaling networks, Cell Communication and Signaling
The interleukin-6-type cytokine Oncostatin M (OSM) activates all three MAPK families: Erk1/2, JNK and p38. Their activation is initiated by phosphorylation of a single tyrosine residue in the cytoplasmic region of the OSM receptor: tyrosine 861. Our work aims at providing a more detailed picture about adaptor/scaffold proteins and small GTPases involved in OSM-mediated MAPK activation.
Gene expression profiling predicts novel targets of the RNA binding protein Musashi1 in the suppression of apoptosis and maintenance of cell proliferation
Poster plus application for short presentation
Stem Cells and Growth Control
A detailed study of the effects of the stem cell translational regulator Musashi-1 with novel mechanistical insights and evidence for an oncogenic function in bladder cancer cells
Effect of hypoxia and oxidized LDL on autophagic and apoptotic responses in THP-1 derived macrophages
Poster presentation
Signaling in Disease
DOES RAS ATTENUATE LRRK2-INDUCED NEURONAL DEGENERATION?
Poster plus application for short presentation
Signaling in Disease
IDENTIFICATION OF NOVEL PROTEINS INTERACTING WITH WNK4 PROTEIN KINASE
Poster presentation
Signaling networks
Development and biological effects of specific inhibitors of SHP-2 protein tyrosine phosphatase
Invited Speaker
Signal Transduction and Drug Discovery
Characterization of mono-ADP-ribosylating poly-ADP-ribose polymerases and unbiased identification of their substrates using protein microarrays
Poster plus application for short presentation
Proteomics, Signalling and Disease
Ectodomain shedding of the type II transmembrane serine protease matriptase-2, the key regulator of iron homeostasis
Poster presentation
Signaling in Disease
Modulation of hepatic insulin signaling in hepatoma cell lines by Prostaglandin E2 (PGE2)
Poster presentation
Signaling in Disease
PGE2 modulates hepatic insulin signaling in hepatoma cell lines.
Regulation of glutathione peroxidase 2 (GPx2) via the Wnt pathway
Poster plus application for short presentation
Signaling in Disease
Role for CSN5 and the COP9 Signalosome in Wnt/β-Catenin Signaling in Colorectal Cancer Cells
Poster presentation
Signaling in Disease
Role of proteoglycans in endothelial deposition of MIF
Poster presentation
Glycobiology and Cell Signalling
Structural and functional characterization of FMIP/THOC5 in DNA damage signaling
Poster plus application for short presentation
Signaling networks
FMIP/THOC5 is a nuclear-cytoplasmic shuttling protein and a member of mammalian THO complex, involved in the processing and transport of pre-mRNA. THOC5 has been identified as a substrate of multiple tyrosine kinases like Bcr-Abl. We are investigating the possible structural and functional effects of THOC5 phosphorylation in DNA damage signaling.
SERCA inhibition forces multidrug-resistant bax/bak-deficient tumor cells into necrosis
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Rheb Signalling towards mTORC1 – Reconsidering the Role of FKBP38 and Design of a Biosensor for Active Rheb
Poster plus application for short presentation
Signaling networks
ERK1/2 activation mediates leptin-induced CXC chemokine production in human airway epithelial cells
Poster plus application for short presentation
Signaling in Disease, Signaling networks, Cell Communication and Signaling
Leptin is a pleiotropic type I cytokine with immunomodulating properties. The present study designates bronchial epithelial cells as novel target cells for leptin action and shows that leptin stimulates the production of the neutrophil chemoattractants CXCL1, CXCL2 and CXCL8 in NCI-H292 cells, which is mediated by ERK1/2 activation.
The APP intracellular domain (AICD) exhibits signal transduction in Alzheimer’s pathophysiology
Poster plus application for short presentation
Proteomics, Signalling and Disease
Within the course of Alzheimer’s disease, APP is proteolytically cleaved by secretases resulting in the generation of sAPPß, ßAmyloid, and AICD (APP intracellular domain). Latter part of the protein was studied in regard to its putative role in signal transduction.
Nerve growth factor (NGF)-mediated survival of human mastocytoma cell line HMC-1.1 in presence of Abl/Kit-tyrosine kinase inhibitor Imatinib is independent of STAT5
Poster plus application for short presentation
Signaling in Disease
Nerve growth factor rescues HMC1.1 cells from Imatinib-induced cell death by mediating STAT3 activation potentially via Erk1/2, PKC and Jak2.
Synthesis and Evaluation of Specific Inhibitors of the Oncogenic Proteine Tyrosine Phosphatase SHP-2
Poster presentation
Signal Transduction and Drug Discovery
DNA binding cooperativity of p53 is essential for apoptosis but not cell cycle arrest
Poster presentation
Signaling in Disease
Role of protein histidine phosphatase in human umbilical vein endothelial cells
Poster plus application for short presentation
other free topics, Signaling in Disease
Structure-function analysis of the ubiquitin-editing and NF-B inhibitory functions of A20
Poster presentation
Structure-function analysis of the ubiquitin-editing and NF-B inhibitory functions of A20
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Changes in the expression of MDR transporters in J774 mouse macrophages (MΘ) selected by chronic exposure to ciprofloxacin (CIP) or moxifloxacin (MXF), 2 fluoroquinolone antibiotics.
Poster plus application for short presentation
Xenobiotics and Signalling
Identification of novel ER-α target genes in breast cancer cells: gene- / cell-selective co-regulator recruitment at target promoters determine response to 17-β-estradiol and tamoxifen
Poster plus application for short presentation
Proteomics, Signalling and Disease
Green tea flavonoid (-)-epigallocatechin-3-gallate (EGCG) exerts both insulin-mimetic and antagonistic effects on FoxO transcription factors.
Poster presentation
Xenobiotics and Signalling
Hepatoma-derived growth factor related protein –3 _ a neuron-specific protein important for neuronal cell differentiation
Poster presentation
Proteomics, Signalling and Disease
Calpain mediates the apoptotic effect of a synthetic sphingosine-1-phosphate analog in postmitotic neurons
Poster presentation
Signal Transduction and Drug Discovery
A novel biological function of the PP2A PR130/B”alpha1 complex in cell migration and adhesion
Poster plus application for short presentation
other free topics, Signaling networks
Tubulin, a new interaction partner of Hepatoma-Derived Growth Factors family members.
Poster presentation
Proteomics, Signalling and Disease
Using the powers of LC/MS-techniques to quantitatively investigate the proteomic profile of myelin isolated from mouse models of metachromatic leukodystrophy (MLD)
Poster presentation
Proteomics, Signalling and Disease
Tom70 phosphorylation is modulating mitochondrial import in response to cellular signaling cascades
Poster presentation
Signaling networks
The role of post-translational acetylation on Cyclophilin A function
Poster plus application for short presentation
other free topics, Biophysical aspects of signal transduction, Proteomics, Signalling and Disease
Here we have used a novel translation system to genetically and site-specifically incorporate N(ε)-acetyl-L-lysine into full-length protein expressed in E. coli. We used this method to elucidate the role of N(ε)-acetylation of lysine K125 in Cyclophilin A function structurally and biochemically.
Artificial Receptors for RGD-containing Glycoproteins
Poster presentation
Signal Transduction and Drug Discovery
Synthetic approach to a new class of ditopic artificial RGD-receptors
Differential impact of zinc signals on p38 MAPK and ERK1/2 activity in T-cells
Poster plus application for short presentation
other free topics
Basolateral targeting of the PDZ domain containing protein FRMPD2 is regulated by armadillo protein p0071 in epithelial cells
Poster plus application for short presentation
Cell Communication and Signaling
Glycosylation pattern of the activating human NK cell receptor 2B4 (CD244)
Poster plus application for short presentation
Glycobiology and Cell Signalling
Double-stranded RNA induces S100 gene expression related to inflammation in HaCaT keratinocytes
Poster plus application for short presentation
Signaling in Disease
Identification of interaction partners of Hepatoma-derived growth factor using affinity-column and mass spectrometry approach
Poster presentation
Proteomics, Signalling and Disease
cAMP counteracts IL-6 signalling
Poster presentation
Signaling networks
Investigation of Membrane Microdomains surrounding Natural Killer Cell Receptors
Poster plus application for short presentation
Signaling networks, Proteomics, Signalling and Disease
A systems biology view of IL-6-induced signal transduction
Poster presentation
Signaling networks
A Novel Mechanism for the Regulation of Gab1 Recruitment to the Plasma Membrane
Poster presentation
Signaling networks
Low concentrations of docosahexaenoic acid but not of oleic acid protect human umbilical vein endothelial cells from apoptotic cell death
Poster presentation
other free topics
Pharmacological modulation of TGFb1 signalling identifies pathways involved in N-cadherin upregulation in vascular smooth muscle cells
Poster presentation
Xenobiotics and Signalling
Our results show that TGFb1 induces the differentiation of primary human vascular smooth muscle cells by Smad2/3-dependent upregulation of the transcription factor Snail1 which results in increased expression of cell-cell adhesion protein N-cadherin and the assembly of N-cadherin/catenin complexes. Our data suggest a role for TGFb1 in promoting intimal fibrogenesis and atherosclerotic plaque formation by differentiation of vascular smooth muscle cells and induction of myofibroblast-dependent processes.
Regulation of p53 expression in hypoxic cancer cells incubated with etoposide
Poster plus application for short presentation
Signaling in Disease
Loss of Cystic Fibrosis Transmembrane conductance Regulator (CFTR) function enhances cytokine production through over-activation of intracellular inflammatory signaling pathways.
Poster plus application for short presentation
Signaling in Disease
Mass spectrometry of a growth factor
Poster plus application for short presentation
Proteomics, Signalling and Disease
ALL-associated JAK1 mutants selectively mediate constitutive activation and hypersensiveness of type I IFN and IL-9 receptor complexes
Poster plus application for short presentation
Signaling in Disease
Mechanism of constitutive and ligand-induced activation of JAK1-binding receptor complexes by ALL-associated JAK1 mutants.
Role of the COP9 Signalosome in Atherosclerosis
Poster plus application for short presentation
Signaling in Disease
Critical role of the transcription factor HNF6 in the development of pancreatic acinar-to-ductal metaplasia
Poster plus application for short presentation
Signaling in Disease
Characterization of the Roco family of proteins
Poster presentation
Signaling in Disease
IL-17 promotes p38 MAPK-dependent endothelial activation enhancing neutrophil recruitment in lung diseases
Poster plus application for short presentation
Signaling in Disease
Lipocalin 2 (LCN2) in liver injury
Poster presentation
Signaling in Disease
Expression and regulation of Lipocalin 2 in liver disease
Directed immobilization of fluorescently labelled cytokines for the analysis of their signal transduction by confocal microscopy
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Effects of X-rays and alpha particle irradiations on gene expression pattern in human lung carcinoma A549 cells
Poster presentation
other free topics
c-Met mediated signalling in hepatocytes protects against cholestatic liver injury and fibrosis progression in mice
Poster presentation
Signaling in Disease
A study about the contribution of the HGF receptor c-Met to hepatocyte protection in a model of chronic cholestatic liver injury in hepatocyte specific conditional c-Met depleted mice
MIF-mediated Activation of the JNK-MAPK Signaling Pathway and Dependence on CXCR4
Poster presentation
Signaling networks
Structural Characterization of the Ligand-Receptor Interface Responsible for MIF/CXCR2-Driven Leukocyte Recruitment
Poster plus application for short presentation
Signaling in Disease, Signal Transduction and Drug Discovery
Differential tyrosine phosphorylation of the STAT5A transcription factor by oncogenic kinases.
Poster plus application for short presentation
Signaling in Disease
The role of the N-terminal domain in dimerization and nucleocytoplasmic shuttling of latent STAT3
Poster plus application for short presentation
Biophysical aspects of signal transduction
Characterization of the murine OSM-inhibitor mOSM-RFP: Fused receptor domains act as high-affinity cytokine-binding proteins
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Purification and biochemical characterization of recombinant NOV and CTGF proteins in eukaryotic expression systems
Poster presentation
Biophysical aspects of signal transduction
Establishing of expression systems for purification of different recombinant CCN proteins
Characterization of gene-targeted murine embryonic stem cells expressing a STAT3-YFP allele
Poster plus application for short presentation
Stem Cells and Growth Control
CSN- and Den1-mediated deneddylation: Another layer of regulation of the ubiquitin proteasome system (UPS)
Poster presentation
Proteomics, Signalling and Disease
A New Player in mTOR Regulation: Introducing FKBP51
Poster presentation
Signaling networks
MAD1 dependent inhibition of apoptosis is followed by decreased stability
Poster presentation
Signaling networks
Characterization of the effects of hydrolyzed pectin in HepG2 and A549 cells
Poster presentation
other free topics
The structure of Nup153ZnF2 and in complex with Ran: A general Ran docking site at the nuclear pore complex
Poster plus application for short presentation
other free topics, Signaling networks, Signal Transduction and Drug Discovery , Biophysical aspects of signal transduction
Cause and effect of lateral mobility of BMP receptors
Poster plus application for short presentation
Signaling networks, Signal Transduction and Drug Discovery , Biophysical aspects of signal transduction
BMP receptors initiate signalling from different origins within the plasma membrane, with different branches of the signal cascades being preferentially initiated from certain origins. We aim to dissect the structural basis of the association of receptor with certain membrane domain and the role of this association for signal initiation, specificity and finetuning.
The phosphorylation at threonine 84 and serine 552 regulates the activity of the NF-kappaB transcription factor RelB
Poster plus application for short presentation
Signaling networks
Analysis of the phosphorylation-dependent structural alterations of the IkappaB-kinase complex
Poster presentation
Signaling networks
Signalling at the gate – phosphorylation of mitochondrial import receptors
Poster plus application for short presentation
Signaling networks, Cell Communication and Signaling
Phosphorylation of the mitochondrial Translocase of the Outer Membrane (TOM) complex by protein kinase CK2 adjusts the mitochondrial protein import machinery to cellular demands.
Chemokine activity induced by Interleukin-6
Poster presentation
other free topics
Activation of STAT proteins by Cytotoxic Necrotizing Factors (CNFs).
Poster presentation
Signal Transduction and Drug Discovery
The redox-active signaler pyocyanin binds to the site of antimalarial phenothiazine drugs in glutathione reductase
Poster presentation
Signal Transduction and Drug Discovery
The bacterial signalling pigment pyocyanin (PYO) and the synthetic drug methylene blue (MB) have many cell-biochemical properties in common. The comparison of the two compounds will guide our studies on MB-based combination therapies against falciparum malaria in West Africa.
TNF-induced necroptosis, a controlled way of cellular explosion
Invited Speaker
Signal Transduction and Drug Discovery
Chaperone-assisted protein folding in health and disease
Invited Speaker
other free topics
Structural changes during nucleotide hydrolysis alter oligomerization state and product ratio of the human guanylate binding protein 1
Poster plus application for short presentation
Biophysical aspects of signal transduction
Charge-reverse and Cross-linking experiments to investigate structural changes and oligomerization behaviour in the course of GTP hydrolysis.
The Differentiation of Adipose Tissue Derived Stem Cells is Affected by Extracellular Nucleotides
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Cellular functions of SorCS1
Poster plus application for short presentation
other free topics
Negative regulation of the EGFR-MAPK cascade by actin-MAL mediated Mig6/Errfi-1 induction
Poster plus application for short presentation
Xenobiotics and Signalling
Hepatoma-derived growth factor – just a prognostic tumor marker?
Poster presentation
Signaling in Disease
Steady-State Analysis of Array-Based Binding Assays
Poster plus application for short presentation
Signaling networks, Proteomics, Signalling and Disease
We propose a mechanistical mathematical model that can be used to estimate the dissociation equilibrium constants from data aquired upon array-based binding assays. A case study on immunobinding is presented and iits applicability to signal transduction and diagnostic approaches are discussed.
Gene transfer of the inositol 5-phosphatase SHIP-1 inhibits proliferation of acute myeloid leukemia cells
Poster plus application for short presentation
Signaling in Disease
The data demonstrate that expression of SHIP-1 can reduce the proliferation of primary acute myeloid leukmia cells
Identification of a novel heteromeric CD74/CXCR4 receptor complex with a role in cytokine/chemokine signaling
Poster presentation
Signaling in Disease, Cell Communication and Signaling
CRE/LOX-ASSISTED CELL TRACKING REVEALS TRANSDIFFERENTIATION OF SMOOTH MUSCLE CELLS DURING ATHEROGENESIS
Poster plus application for short presentation
Signaling in Disease
CTS-1: a dominant-positive p53-derived synthetic tumor suppressor and putative tool for developing a p53-based gene therapy in human malignant gliomas
Poster presentation
Signaling networks
FERM domain mediated interaction of the protein tyrosine phosphatase PTP-BL with the Serological Defined Colon Cancer Antigen 3 (SDCCAG3)
Poster plus application for short presentation
Cell Communication and Signaling
Checkpoint Recovery and Cancer
Invited Speaker
Stem Cells and Growth Control
Functional and dynamic analysis of the NADPH oxidase assembly during phagocytosis
Poster plus application for short presentation
Cell Communication and Signaling
Scatter factor receptor/c-met signaling in dendritic cells plays an essential role in contact hypersensitivity responses
Poster plus application for short presentation
Cell Communication and Signaling
Basal and atrophy stimuli-induced expression of Atrogin-1/MAFbx is dependent on GSK-3β activity in C2C12 myotubes
Poster presentation
Signal Transduction and Drug Discovery
ISG15 conjugation of proteins
Poster presentation
other free topics
ISG15 conjugation of proteins
Poster presentation
other free topics
Function and regulation of PML nuclear bodies
Poster presentation
other free topics
Function and regulation of PML nuclear bodies
Poster presentation
other free topics
RNA-interference based screen identifies new factors important for NF-kappaB activation and termination.
Poster plus application for short presentation
Signaling networks
Automatic Relative Immunofluorescence - A microscopy to investigate signaling pathway underlying pain sensitisation
Poster presentation
Signaling in Disease
Real time Reporter Gene Assay based on Gaussia luciferase as a novel tool for the analysis of signal transduction pathways
Poster presentation
Signal Transduction and Drug Discovery
A novel reporter gene assay is presented based on a secreted luciferase
Role of the Transmembrane Helix of Plateled-derived Growth Factor Receptor beta
Poster presentation
Biophysical aspects of signal transduction
Different interactions of Rassf5 in the apoptotic pathway of Ras
Poster presentation
Biophysical aspects of signal transduction
Asymmetric chromosome segregation in dysplastic megakaryocytes in myelodysplastic syndromes
Poster plus application for short presentation
other free topics
The aim of our study was to elucidate chromosome distribution within dysplastic polynucleated megakaryocytes (MKs) of myelodysplastic syndromes (MDS). We performed sequential multilocus FISH on bone marrow smears to trace the distribution of 4 different chromosomes. By using this approach, we evaluated 60 MKs by 3D-microscopy. Varying chromosome numbers were detected within the separate nuclei of all polynucleated MKs, indicating an asymmetrical segregation of chromosomes in dysplastic, polynucleated MKs of MDS. This study provides the basis for further examination of the regulatory mechanisms involved in endomitosis and subsequent polynucleation of MKs in MDS.
Influence of the conserved cysteines on the membrane structure of bovine papillomavirus E5 oncoprotein
Poster presentation
Biophysical aspects of signal transduction
Myocardial G-protein-mediated Signaling in Morphine-dependent Rats
Poster presentation
other free topics, Proteomics, Signalling and Disease
NLRC5 a new member of the NLR family is involved in regulating innate immune responses
Poster presentation
Signaling networks
INDUCTION OF β-CATENIN SIGNALING AND STIMULATION OF MYOTUBE FORMATION BY WNT-3A OR GSK-3β INACTIVATION
Poster presentation
Signaling in Disease
Skeletal muscle atrophy contributes significantly to disability, increased morbidity and mortality in chronic obstructive pulmonary disease. Reversal of muscle atrophy is an important therapeutic strategy. Wnt-3a is shown to improve both myoblast fusion and myotube formation. Understanding muscle regeneration will aid in development of drugs targetted at improving muscle regeneration.
Molecular steps involved in cytokine-induced damage of OPCs
Poster presentation
Signaling in Disease
THE MANY FACES OF THE SOCS BOX
Poster plus application for short presentation
Proteomics, Signalling and Disease
Gel-free proteomic analysis of proteins interacting with a HIV-1 intragenic regulatory DNA sequence
Poster presentation
Proteomics, Signalling and Disease
REGULATION OF SON OF SEVENLESS (SOS) BY EZRIN-ACTIN
Poster plus application for short presentation
Cell Communication and Signaling
AMP-activated protein kinase activation, a therapeutic approach to treat insulin resistance in the heart
Poster plus application for short presentation
Signaling in Disease
We showed that the activation of AMPK increases insulin sensitivity in the heart by overstimulating PKB signaling. This correlates with the inhibition of the IRS1-mediated negative feedback loop.
Activation of the somatostatin receptor Sst2 enhances rapamycin’s antiproliferative action by inhibiting rapamycin-induced IRS-1 and Akt activation
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Interplay of the COP9 signalosome, USP15 and other associated proteins in vivo
Poster presentation
Proteomics, Signalling and Disease
Aurora-A is associated with aneuploidy and multipolar mitoses in chromosomal-, but not Microsatellite instable sporadic colorectal cancers
Poster plus application for short presentation
other free topics
Aneuploidy is a key event in the carcinogenesis of colorectal cancer (CRC). This study investigated the association of the centrosomale kinase Aurora-A with aneuploidy and multipolar mitosis in chromosomal- (CIN) and microsatellite- (MIN) instable CRC. Aurora-A or Aurora-A with PLK1 is linked to multipolar mitoses in aneuploid CIN-type CRCs. In diploid MIN-type CRC, multipolar mitoses occur only in the presence of high PLK1 levels and activity and forced triggering of the spindle checkpoint. Aneuploidy may therefore depend on combination of Aurora-A and PLK1 activation with spindle assembly checkpoint control.
Nanoparticle-induced cell signalling events as targets for a strategy of molecular prevention
Poster plus application for short presentation
Signaling in Disease
The work describes the activation of pathogenic signalling pathways by inhalable nanoparticles in lung epithelial cells on the level of membrane receptors and lipid rafts. Pretreatment with macromolecule-stabilizing compatible solutes in vitro and in vivo prevents these signalling events as well as the respective endpoints.
Probing CNTF receptor stoichiometry using fluorescent cross-correlation spectroscopy
Poster plus application for short presentation
Biophysical aspects of signal transduction
Phosphocholine in Parasitism - Biosynthesis and immunological implications of PC-epitopes
Poster plus application for short presentation
The atomic view of signaling
A specific interaction between two residues in the pseudokinase domain of JAK2 is necessary for the constitutive activation of JAK2 V617F
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Targeting signal transduction cascades in disease by novel small molecule nucleo-cytoplasmic transport inhibitors
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Deregulated signalling cascades ultimately excecute their pathobiological consequences by the modulation of genetic programs. The biological function of disease relevant transcription factors depend on regulated nucleo-cytoplasmic transport, which is controlled by specific signals and transport factors. Hence, the rational modulation of nucleo-cytoplasmic transport by small molecules would not only aid in dissecting signal transduction cascades but also holds a promising therapeutic potential. Consequently, we developed high-content cellular translocation biosensors and identified chemical nuclear export inhibitors interfering with the pathobiological activity of signalling cascades and hence, may be exploitable in anti-cancer strategies.
Expression of estrogen receptor alpha increases leptin-induced STAT3 activity in breast cancer cells
Poster plus application for short presentation
Signaling in Disease
The proinflammatory cytokines IFNγ and TNFα induce shedding of endothelial and epithelial surface molecules via the upregulation of ADAM17 activity
Poster presentation
other free topics
The role of CD95/APO-1/Fas glycosylation in the sensitivity and resistance of cells towards death receptor-induced apoptosis
Poster plus application for short presentation
Glycobiology and Cell Signalling
Interplay of ZIP kinase, AATF and TSG101 in Androgen Receptor-mediated Transcription
Poster presentation
other free topics
Regulated intramembrane proteolysis of transferrin receptor 1
Poster presentation
Cell Communication and Signaling
The acrosome reaction is regulated by Cdc42.
Poster presentation
Signaling networks
Effect of mitogens on the growth of stem cell-like brain tumor cells.
Poster plus application for short presentation
Stem Cells and Growth Control
Receptor tyrosine kinase expression in cell populations derived from malignant human brain tumors
Poster presentation
Signaling in Disease
Activation of the cardiac mTOR/p70S6K pathway by leucine requires PDK1 and correlates with PRAS40 phosphorylation
Poster plus application for short presentation
other free topics
Amino acids like leucine are potent signalling regulator. Leucine is known to regulate p70S6K pathway. We showed, here, that PDK1, known to mediate insulin effect, is also invovled in leucine action.
MIGRATION OF HUMAN TUBULAR EPITHELIAL CELLS ON DIFFERENT EXTRACELLULAR MATRICES: MODULATION BY RHO-KINASE INHIBITORS
Poster plus application for short presentation
Cell Communication and Signaling
Our data show the importance of the extracellular matrix for the migration of tubular epithelial cells, also evident when the cells were stimulated with TGF-beta. By contrast, inhibition of Rho-kinases stimulated migration in a matrix independent manner. Pharmacologic inhibition of Rho-kinases may thus favor repair processes by increasing the migratory capacity of tubular epithelial cells
The Differential Regulation of Connective Tissue Growth Factor (CTGF) by Hypoxia in Endothelial and Epithelial Cells Depends on FoxO3a
Poster plus application for short presentation
Signaling networks
FoxO transcription factors have been previously described as context-dependent regulators of cell growth and differentiation. In this study, we were able to link the transcription factor FoxO3a to the cell type-specific upregulation of CTGF in endothelial cells and demonstrate its relevance for the differential regulation of CTGF by hypoxia in endothelial and epithelial cells.
A-769662, the new AMP-activated protein kinase activator, is a potent inhibitor of the phenylephrine-induced cardiac hypertrophy
Poster plus application for short presentation
Signaling in Disease
We studied the putative action of A-769662, the new AMPK activator, on cardiac hypertrophy.
Modulation of gene expression and tumour cell growth by redox modification of Signal Transducer and Activator of Transcription 3.
Poster plus application for short presentation
Signaling in Disease
Molecular and cellular analysis of STAT3 function in relation to intracellular redox changes involving DNA interactions, gene expression studies, cell cycle analysis.
Disturbance of CMP-Sia homeostasis results in renal failure
Poster plus application for short presentation
Glycobiology and Cell Signalling
Characterization of Mab 5G8, a monoclonal antibody directed against Rab1
Poster presentation
other free topics
Structural Analysis of the Interaction of Vinculin with Actin Filaments
Poster presentation
Biophysical aspects of signal transduction
Activation of AMP-activated protein kinase, a putative approach in cardiac cell therapy
Poster plus application for short presentation
Stem Cells and Growth Control
IL-10 induced inhibition of dendritic cell maturation and function is mediated by STAT3
Poster presentation
Signaling in Disease
CELLULAR SENESCENCE OF S100A6 DEFICIENT MOUSE NIH 3T3 FIBROBLASTS
Poster presentation
other free topics
Expression of the Adiponectin Receptor 1 N-Terminus
Poster presentation
Signaling in Disease
Combination of ChIP and in situ biotinylation for functional studies on STAT3
Poster presentation
Signaling in Disease
The Role of Phosphorylation of the Neural Cell Adhesion Molecule (NCAM) During Neurite Outgrowth
Poster presentation
Cell Communication and Signaling
Cell cycle dependent phosphorylation of ING5
Poster presentation
other free topics
Establishing a FACS based FRET analysis for detecting protein-protein interactions
Poster presentation
Signaling in Disease
Signaling induced by endogenous ligand independent autoimmune activation of Angiotensin type 1- and Endothelin Type A-receptor in microvascular endothelial cells
Poster plus application for short presentation
Signaling in Disease
Identification of a Precisely Configured Dimeric Interface for the Active G-CSF-R: Receptor Tetramerization Does Not Induce Differentiation
Poster plus application for short presentation
The atomic view of signaling
Identification of one dimeric G-CSF-R interface as the candidate for the active G-CSF-R transmembrane-cytosolic domain conformation, by use of coiled coils to impose different dimeric and tetrameric orientations.
SWAP-70 regulates IL4R signaling to the immunoglobulin heavy chain IgE locus
Poster presentation
other free topics, Signaling networks
A p38 MAP-kinase cascade sustaining membrane traffic and cytoskeletal organisation in the C.elegans intestine.
Poster plus application for short presentation
other free topics, Signaling in Disease, Signaling networks
Which signalling regulators maintain polarity and thus integrity of epithelial cells? We conducted an in vivo RNA interference (RNAi) screen in the intestine of the nematode C.elegans for genes relevant for cell polarity and polarised membrane traffic. Among the screen hits were several components of a p38 MAP (MAPK) kinase cascade. We will present our insights into its novel relevance in maintaining intestinal integrity. Upon silencing any of three MAP kinases (a MAP3K, a MAP2K, and a p38 MAPK) or three specific transcription factors, the morphology of the intestinal tissue becomes strongly affected. Both cytoskeletal organisation and membrane transport are changed, causing endosomes to scatter and membrane cargo to accumulate in late endosomes. In consequence, the intestinal lumen is irregular and frequently exhibits cysts. The identical phenotype patterns of each of the MAP kinases and transcription factors suggest that the components act in a hierarchical, linear cascade. Currently, we investigate the signalling network and downstream factors of this MAP kinase cascade to specify its novel roles in enterocytes.
Mutual influence of Btk and SHIP1 in mast cells: dependence and compensation
Poster plus application for short presentation
Signaling networks
Host receptor tyrosine kinase activation by the Listeria protein InlB
Invited Speaker
The atomic view of signaling
We describe the structure of the dimeric, signaling active complex between the Listeria invasion protein InlB and the human receptor tyrosine kinase Met.
Crystal structure of the catalytic domain of the human protein tyrosine phosphatase SHP2
Poster presentation
Biophysical aspects of signal transduction, The atomic view of signaling
Acidosis-induced activation of ERK1/2 and p38 kinases in rat prostate cancer cells (AT1)
Poster plus application for short presentation
Signaling in Disease
Inhibitors of Cyclin Dependent Kinase 9
Poster plus application for short presentation
Signal Transduction and Drug Discovery
As a general regulator of transcription, CDK9 is being discussed as a therapeutic target for treatment of diseases like inflammation, HIV, cancer and cardiac hypertrophy.We have developed different series of highly specific CDK9 inhibitors. With cellular and in vivo data, we validated CDK9 as a target for HIV treatment and inflammatory diseases. At effective concentrations, specific CDK9 inhibitors did not result in any detectable toxicity.
Probing the interaction of mTOR FKBP-rapamycin binding domain with phospholipids by NMR spectroscopy
Poster presentation
Biophysical aspects of signal transduction, The atomic view of signaling
Hepatocyte-specific NEMO deletion in mice leads to oxidative stress lethality during liver regeneration
Poster plus application for short presentation
Signaling in Disease, Signal Transduction and Drug Discovery
NEMO is the regulatory subunit of the IKK complex activating the transcription factor NF-κB. Hepatocyte-specific NEMO knock-out (NEMOΔhepa) animals show a complete inhibition of NF-κB signalling in parenchymal cells leading to a severe phenotype. Animals develop spontaneous hepatocyte apoptosis counterbalanced by proliferation, progressing to hepatocellular carcinoma. As NF-κB is involved in early events of hepatocyte proliferation, we studied the impact of hepatocyte-specific NEMO deletion on liver regeneration.
The anti-inflammatory annexin A1 peptide Ac1-25 inhibits pro-inflammatory activation of monocytes through JAK/STAT/SOCS signaling
Poster presentation
Signaling networks
The chicken lysozyme GAS41 origin mediates episomal replication of a SV40 origin-containing vector in myelomonocytic HD11 cells
Poster presentation
other free topics
Fluorophore binding aptamers as a tool for visualization of RNA
Poster presentation
other free topics
Identification and Characterization of Interaction Partners for Metabotropic Glutamate Receptors Expressed in the Retina
Poster presentation
Signal Transduction and Drug Discovery
Deciphering the autophagic regulatory network by quantitative drug screening
Poster plus application for short presentation
Signaling in Disease
We have established a quantitative method to detect autophagic activity in breast cancer cells. Using this method we have investigated the underlying signaling network under various conditions.
EGFL7 is the first secreted inhibitor of Notch signaling in neural stem cells
Poster plus application for short presentation
Stem Cells and Growth Control
Glioblastomas express PTPIP51, an interacting partner of 14-3-3ß
Poster presentation
other free topics
Structure determination of metabotropic glutamate receptor C-termini
Poster presentation
Signal Transduction and Drug Discovery
Atomic force spectroscopy measures induced structural changes in Sensory Rhodopsin II
Poster presentation
other free topics, Biophysical aspects of signal transduction
Sequential steps of leukocyte recruitment via the transmembrane chemokine CX3CL1
Poster plus application for short presentation
Cell Communication and Signaling
A Fluorescence spectroscopic approach to quantify the binding characteristics of the intracellular domain of Crumbs with PDZ domain-containing proteins in Drosophila melanogaster
Poster plus application for short presentation
other free topics, Signaling networks, Biophysical aspects of signal transduction
Single molecule studies of the conformational dynamics of translation initiation complex
Poster presentation
Biophysical aspects of signal transduction
The influence of the branched-chain fatty acids pristanic acid and Refsum disease-associated phytanic acid on mitochondrial functions and calcium regulation of hippocampal neurons, astrocytes, and oligodendrocytes
Poster plus application for short presentation
Signaling in Disease
Branched-chain fatty acids pristanic and phytanic acid accumulate in inherited pertoxisomal disorders. We investigate the mechanism of toxicity of these fatty acids in brain cells.
Mapping of cysteine bonds within the extracellular domain of the inhibitory glycine receptor
Poster presentation
Biophysical aspects of signal transduction, Proteomics, Signalling and Disease
NF-kB family member p50 impairs the development of M1 and protects the liver from infection-associated inflammatory injury
Poster plus application for short presentation
Signaling in Disease
Single molecule FRET reveals novel dynamic structure and stoichiometry of L27 domain-mediated polarity complexes formed by Drosophila Sdt/DPATJ/DLin-7
Poster plus application for short presentation
other free topics, Signaling networks, Biophysical aspects of signal transduction
Connection between Vav3, EGFR Signaling and Responsiveness to Cetuximab in Tumor Cells
Poster presentation
Signaling networks
Investigation the Functional Role of the Protein Tyrosine Kinase Pyk2 in Lung Cancer
Poster presentation
Signaling networks
Characterization of the Binding of Klotho to Different Cell Lines and its Cellular Signaling
Poster presentation
Signaling networks
EGFR phosphorylation: Simultaneous detection of eight phosphorylation sites upon inhibitor treatment
Poster plus application for short presentation
Signal Transduction and Drug Discovery
The role of MIF in liver fibrosis
Poster presentation
Signaling in Disease
Molecular design and targeted cellular delivery of engineered human deoxycytidine kinase to enhance metabolic activation of nucleoside analog prodrugs and improve anticancer therapy.
Poster plus application for short presentation
Signal Transduction and Drug Discovery
Engineering of a nucleoside kinase to enable it to exclusively phosphorylate non-physiological L-nucleoside analogs, and delivery of the enzyme to tumour cells via EGFR-specific antibodies.
Up-regulation of the immediate-early gene product RhoB by the cytotoxic necrotizing factor 1 from E. coli
Poster plus application for short presentation
Signaling in Disease
Colocalization of the Cytosolic Protein Tyrosine Kinase Pyk2 with Autophagosomes in Non-Small Cell Lung Cancer (NSCLC) Cells
Poster presentation
Signaling networks
IL31: signaling, target genes and biological function in the skin
Poster presentation
Signaling networks
Functional Role of Rac2 in B-Cell-Antigen-Receptor-mediated Activation of Phospholipase C-gamma2 in DT40 B Cells
Poster plus application for short presentation
Biophysical aspects of signal transduction
Identification of proteins and phosphorylations involved in L-LTP
Poster presentation
Proteomics, Signalling and Disease
STRUCTURE-ACTIVITY RELATIONSHIPS OF HUMAN NPFF1 RECEPTOR
Poster presentation
Signal Transduction and Drug Discovery
Single molecule fluorescence spectroscopy of DNA Polymerase conformational dynamics during catalysis
Poster plus application for short presentation
other free topics
The conformational changes of DNA Polymerases during catalysis were analysed using high resolution single molecule fluorescence spectroscopy.
Taking Advantage of a Low Populated Excited State in the Ras Protein for NMR-Assisted Inhibitor Design
Poster presentation
Signal Transduction and Drug Discovery
Identification and stabilization of an excited conformational state of Ras
Poster plus application for short presentation
Biophysical aspects of signal transduction
Methodologies to investigate and modulate conformational equilibria as well as a interesting structure from therapeutical view is presented
Structure and function of new oligomeric protein species of human PAPS synthetases
Poster plus application for short presentation
Biophysical aspects of signal transduction
Functional characterisation of parvulin proteins in chicken DT40 cells
Poster presentation
Cell Communication and Signaling
Effects of MIF and other chemokines on the angiogenic potential of endothelial cells and its progenitors
Poster presentation
Proteomics, Signalling and Disease
PPARalpha activation not only enhances GRalpha-mediated pro-inflammatory gene repression, but also prevents glucocorticoid-mediated hyperinsulinemia: a double gain.
Poster presentation
Signal Transduction and Drug Discovery
STRUCTURAL BASIS OFR RAGE-S100B INTERACTION
Poster plus application for short presentation
The atomic view of signaling
RAGE (Receptor for Advanced Glycation Endproducts) is a member of the immunoglobulin superfamily of cell-surface receptors. They share key structural properties and are involved in the activation of cells of the immune system and the central nervous system. Hyper-activation of these receptors results in chronic inflammation , disturbed immune response and cancer.
High-speed intravital microscopy visualizes the uptake of nanocrystal-labelled lipoproteins in liver cells in vivo
Poster plus application for short presentation
other free topics
αA-crystallin and αB-crystallin, newly identified interaction proteins of protease-activated receptor-2 (PAR-2), protect astrocytes from cell death
Poster plus application for short presentation
Signaling in Disease
In this work a novel neuroprotective signaling pathway is presented.
Characterization of a clathrin-dependent receptor-mediated internalization pathway of MIF
Poster presentation
Cell Communication and Signaling
Single-molecule FRET reveals dNTP-dependent conformational transitions in the fingers subdomain of Klentaq1
Poster presentation
Biophysical aspects of signal transduction
Phosphorylation of Tip60 by GSK-3 determines the decision for apoptosis induction by p53
Poster plus application for short presentation
Signaling in Disease
We show that GSK-3, by phosphorylation of serine-86 of the p53 acetyltranserase Tip60, is required for the pro-apoptotic activity of p53, inducing the BCL-2 family member PUMA.
Is MAGEA1 an oncogene?
Poster presentation
Stem Cells and Growth Control
MAGEA1-expressing mice will be monitored for tumor development
Maged2 is involved in DNA damage responses
Poster presentation
Stem Cells and Growth Control
CHAPERONING OF SIGNAL TRANSDUCTION BY THE HSP90 MACHINE
Poster plus application for short presentation
Biophysical aspects of signal transduction, The atomic view of signaling
We monitor chaperoning of kinases by NMR spectroscopy. We use methyl specific labelling to characterise those 200 KDa complexes at atomic resolution.
Fluorescence spectroscopy to study membrane transport, leakage and fusion
Invited Speaker
other free topics
TOR signaling and control of cell and animal growth
Invited Speaker
Stem Cells and Growth Control
Mapping of TLR TIR-TIR interactions via MAPPIT
Poster plus application for short presentation
The atomic view of signaling
We study the molecular details of interactions between TIR domains in the TLR signaling cascade. TIR-TIR interactions are studied via the mammalian two hybrid system MAPPIT. The effect of mutations on these interactions is mapped on models of the TIR domains to build models for the TIR signaling complex.
Caspase-1 targets the TLR adaptor Mal at a crucial TIR-domain interaction site
Poster presentation
Signaling in Disease
Cell free expression of G-protein coupled receptors
Poster plus application for short presentation
Biophysical aspects of signal transduction
GPCR, Dopamin receptor D1, cell free expression
Application of (quantitative) proteomics strategies to identify the plasma membrane anchoring protein(s) of Epac1 and additional Epac1-interacting proteins
Poster presentation
Signaling networks, Proteomics, Signalling and Disease
Selectivity of GEF/G-protein interaction
Poster presentation
other free topics
Rover: a tool to visualize and validate quantitative proteomics data from different sources
Poster presentation
Proteomics, Signalling and Disease
Rover is a program to facilitate the manual validation of quantitative proteomics data.
Peptizer: a rule-based bioinformatics tool to select suspicious peptide identifications
Poster presentation
other free topics
KinaseSwitch: A technology platform for the inducible and reversible inhibition of kinases in adult mice
Poster presentation
Signal Transduction and Drug Discovery
Signal transfer from receptor to G protein: Insights from spectroscopic and structural studies on rhodopsin
Invited Speaker
Biophysical aspects of signal transduction
BRAF and RAS signalling in melanoma: therapeutic implications
Invited Speaker
Signaling networks
STAT3 is a key player in oncogenesis mediated by the HCMV encoded GPCR US28
Poster presentation
other free topics
G proteins activated by dimerization
Invited Speaker
The atomic view of signaling
Phosphorylase and the Origin of Cellular Regulation by Protein Phosphorylation
Invited Speaker
other free topics
Lipids and sugars in Mycobacterium host-pathogen interaction
Invited Speaker
Glycobiology and Cell Signalling
The cell wall of pathogenic Mycobacteria is a complex composite of (glyco)lipids, with important roles in host-pathogen interaction. We study the importance of these compounds through mutagenesis of the relevant pathways.
http://www.contoo.de/en_US/congress/papers/id/47/c_cult/en_US/lyt/c/print/1