MALDI in-situ mass spectrometry of renal sulfatides

Christian Marsching¹, Paula Stettner², Dr. Matthias Eckhardt³, Prof. Hermann-Josef Gröne⁴, Prof. Roger Sandhoff⁵, Prof. Carsten Hopf⁶

1 Center for Applied Research in Applied Biomedical Mass Spectrometry (ABIMAS), Mannheim; Lipid Pathobiochemistry, DKFZ, Heidelberg; Instrumental Analysis and Bioanalysis, Department of Biotechnology, University of Applied Sciences, Mannheim

2 Cellular and Molecular Pathology, German Cancer Research Center (DKFZ), Heidelberg

3 Institute of Biochemistry and Molecular Biology, University of Bonn

4 Cellular and Molecular Pathology, German Cancer Research Center (DKFZ), Heidelberg

5 Center for Applied Research in Applied Biomedical Mass Spectrometry (ABIMAS), Mannheim; Instrumental Analysis and Bioanalysis, Department of Biotechnology, University of Applied Sciences, Mannheim; Lipid Pathobiochemistry, DKFZ, Heidelberg

6 Instrumental Analysis and Bioanalysis, Department of Biotechnology, University of Applied Sciences, Mannheim, Germany

Abstract

Sulfatides, a class of acidic glycosphingolipids, are highly expressed in mammalian myelin and in kidney. These molecules are known to be important components for membrane stability and furthermore for osmotic stability in renal cells. Using MALDI in situ mass spectrometry, we analyzed the tissue-distribution of sulfatides with regard to their carbohydrate head group and their ceramide anchor composition. Currently, information on the latter is a unique feature of this technique. Utilizing different genetic mouse models of sulfatide metabolism, on the one hand, we were able to validate the specificity of our mass spectrometry signals; on the other hand, we successfully characterized different relative turnover rates of these molecules in relation to their renal localization.

References

Anal Bioanal Chem. 2011 Jul;401(1):53-64. Epub 2011 Feb 27.

Imaging of complex sulfatides SM3 and SB1a in mouse kidney using MALDI-TOF/TOF mass spectrometry

DOI^®: 10.3288/contoo.paper.1396