Molecular Origami: protein folding and misfoldng in health and disease.

Achieving correct protein folding and quality control is essential for normal cellular function. The accumulation of misfolded proteins is emerging as central to a wide range of disease states, including many neurodegenerative disorders such as Huntington’s and Prion Disease. Molecular chaperones are a diverse family of enzymes that monitor and maintain all aspects of protein homeostasis. The role of chaperones in these diverse functions will be discussed.

Chaperones assist the folding of newly translated and stress-denatured proteins, as well as affects protein quality control. Systems approaches identified a chaperone network linked to the protein synthesis apparatus assists protein biogenesis. The emergence of this translation-linked chaperone network likely underlies the elaborate co-translational folding process necessary for the evolution of larger multidomain proteins characteristic of eukaryotic cells. A stress-inducible chaperone network protects cells from environmental stress and assists quality control. These chaperones also communicate with the ubiquitin-proteasome pathway to clear misfolded proteins from the cell. Protein quality control in the eukaryotic cytosol relies on the sequestration of misfolded cytosolic proteins in specific quality control compartments. Our studies of chaperone function provide a framework to understand the link between protein misfolding and human disease.