Biological effects of copper complexes on human colon carcinoma and mouse embryonic fibroblast cell lines

Katarína Koňariková¹, Lucia Laubertová², Lucia Andrezálová³, Helena Gbelcová⁴, Ľubomír Danišovič⁵, Daniel Böhmer⁶, Ingrid Žitňanová⁷, Zdeňka Ďuračková⁸

1 Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Faculty of Medicine, Sasinkova 2, 813 72, Bratislava, Slovak Republic

2 Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Faculty of Medicine, Sasinkova 2, 813 72, Bratislava, Slovak Republic

3 Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Faculty of Medicine, Sasinkova 2, 813 72, Bratislava, Slovak Republic

4 Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Biology and Genetics, Sasinkova 4, 813 72 Bratislava, Slovak Republic

5 Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Biology and Genetics, Sasinkova 4, 813 72 Bratislava, Slovak Republic

6 Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Biology and Genetics, Sasinkova 4, 813 72 Bratislava, Slovak Republic

7 Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Faculty of Medicine, Sasinkova 2, 813 72, Bratislava, Slovak Republic

8 Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Faculty of Medicine, Sasinkova 2, 813 72, Bratislava, Slovak Republic

Abstract

Biological effects (cytotoxicity and genotoxicity) of two copper complexes (ZK1 - [Cu(N-salicylidene-L-glutamato)(H2O)2].H2O and MK1 - [Cu(N-salicylidene-5-methyl-L-glutamato)(H2O)].H2O) were analyzed in vitro by experiments on two cell lines, namely human colon carcinoma HT-29 and mouse embryonic fibroblast NIH-3T3.
Both cell growth and viability were assessed by direct counting of 0.4% trypan blue dye-excluding cells after 24, 48 and 72 hours cells cultivation with or without copper complexes. At the highest tested concentration an acute cytotoxic effect on HT-29 cells was observed in shorter time after treatment with ZK1 compared to MK1.
Genotoxicity of copper complexes was investigated by single-cell gel electrophoresis (comet assay). Both tested complexes induced DNA breaks formation and their amount increased proportionally with increasing tested concentrations. Notable differences were also observed between the sensitivity among the individual cell lines.

Acknowledgement: This project was supported by Rozum a zdravie.

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DOI^®: 10.3288/contoo.paper.1476